Cytokine production was studied in thyroid fine needle aspirates and peripheral blood and the production of interferon-gamma by peripheral blood mononuclear cell(PBMC) culture in response to interleukin-2(IL-2) stimulation was also studied from
patients
with hyperthyroidism, non toxic goiter, thyroid nodule. The expression of glycer aldehyde 3-phosphate dehydrogenase(GAPDH). Interleukin-1β(IL-1β), IL-2, interleukin-8(IL-8), platelet- derived growth factor-A(PDGF-A) and interferon-gamma(IFN-γ)
chain
was assessed by RT-PCR(reverse transcriptase polymerase chain reaction) in fine needle aspirates of thyroid and peripheral blood mononuclear cell: the samples were obtained from 7 patients with hyperthyroidism. 6 patients with non toxic goiter, 7
patients with thyroid nodule. A dose of IL-2(25 U/ml) was utilized to induce IFN-γproduction by PBMC from all patients.
@ES The results were as follows:
@EN 1) In case of cytokine expression of fine-needle aspirates. GAPDH and IL-1β, IL-8 were expressed highly but IFN-γ, IL-2 were not expressed in hyperthyroidism and non-toxic goiter, thyroid nodule. PDGF-A was expressed in hyperthyroidism and
thyroid
nodule but not in non toxic goiter.
2) In case of cytokine expression of PBMC, GAPDH, IL-1βwere expressed in hyperthyroidism and non toxic goiter, thyroid nodule and highly expressed after Il-2 stimulation than before. But PDGF-A was more expressed in non toxic goiter and thyroid
nodule
than hyperthyroidism. Also, IFN-γwas less expressed in thyroid nodule than hyperthyroidism and non toxic goiter.
3) The incremental in IFN-γvalue in supernatants of PBMC culture was-significantly higher in patients with nn toxic goiter than that in PBMC from hyperthyroidism and thyroid nodule(p<0.05).
Therefore it seems that the cytokine production was found in hyperthyroidism and non toxic goiter and thyroid nodule. There were variability in their distribution each other, in general, higher expressed in hyperthyroidism than non toxic goiter,
And
RT-PCR Method that employed sho(?)ld be sufficiently sensitive to permit the analysis of cytokine gene expression in fine needle aspiration biopsies from patients with thyroid disease (J Kor Soc Endocrinol 10:13~25, 1995).
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